1. About how many cells were plated to count His+ revertant colonies?
How was total cell number determined?
I know that the mutation freq was out of 10^7 survivors. So they had to measure the total cell number and come up with 10^7 survivors? But I'm not sure how they got this exact number of 10^7 survivors?
Then to measure His+ revertants I would think they need to plate the same concentration of cells as they did to measure the total cell count?
1. The problem statement, all variables and given/known data
2. Relevant equations
3. The attempt at a solution
How was total cell number determined?
I know that the mutation freq was out of 10^7 survivors. So they had to measure the total cell number and come up with 10^7 survivors? But I'm not sure how they got this exact number of 10^7 survivors?
Then to measure His+ revertants I would think they need to plate the same concentration of cells as they did to measure the total cell count?
1. The problem statement, all variables and given/known data
2. Relevant equations
3. The attempt at a solution
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